[ ⁶⁴Cu]Cu-Albumin Clearance Imaging to Evaluate Lymphatic Efflux of Cerebrospinal Space Fluid in Mouse Model.

ABSTRACT:

Purpose

Clearance of brain waste in the cerebrospinal fluid (CSF) through the meningeal lymphatic vessels (mLV) has been evaluated mostly through the fluorescent imaging which has inherent limitations in the context of animal physiology and clinical translatability. The study aimed to establish molecular imaging for the evaluation of mLV clearance function.

Methods

Radionuclide imaging after intrathecal (IT) injection was acquired in C57BL/6 mice of 2-9 months. The distribution of [^99mTc]Tc-diethylenetriamine pentaacetate (DTPA) and [⁶⁴Cu]Cu-human serum albumin (HSA) was comparatively evaluated. Evans Blue and [⁶⁴Cu]Cu-HSA were used to evaluate the distribution of tracer under various speed and volume conditions.

Results

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SUBMITTER: Sarker A 

PROVIDER: S-EPMC9123114 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature