Development of β-carotene, lysine, and tryptophan-rich maize (Zea mays) inbreds through marker-assisted gene pyramiding.

ABSTRACT: Maize (Zea mays L.) is the leading cereal crop and staple food in many parts of the world. This study aims to develop nutrient-rich maize genotypes by incorporating crtRB1 and o2 genes associated with increased β-carotene, lysine, and tryptophan levels. UMI1200 and UMI1230, high quality maize inbreds, are well-adapted to tropical and semi-arid regions in India. However, they are deficient in β-carotene, lysine, and tryptophan. We used the concurrent stepwise transfer of genes by marker-assisted backcross breeding (MABB) scheme to introgress crtRB1 and o2 genes. In each generation (from F₁, BC₁F₁-BC₃F₁, and ICF₁-ICF₃), foreground and background selections were carried out using gene-linked (crtRB1 3'TE and umc1066)

SUBMITTER: Chandrasekharan N 

PROVIDER: S-EPMC9123160 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature