Dataset Information

Characterization and functional validation of β-carotene hydroxylase AcBCH genes in Actinidia chinensis.

ABSTRACT: Carotenoids are the pigment substances of yellow-fleshed kiwifruit, and among them β-cryptoxanthin has only been detected in the brighter yellow-fleshed variety 'Jinshi 1'. β-Carotene hydroxylase (BCH) catalyzes the formation of β-cryptoxanthin and zeaxanthin, but its molecular characteristics and functions have not been fully explained. Here we isolated two β-carotene hydroxylase genes, AcBCH1 and AcBCH2 from kiwifruit (Actinidia chinensis), and their relative expression levels exhibited a close correlation with the content of β-cryptoxanthin. AcBCH1 catalyzed the formation of β-cryptoxanthin when transformed into β-carotene-accumulating yeast cells. Moreover, silenced expression of AcBCH1 in kiwifruit caused decreases in the contents of zeaxanthin, lutein, and β-cryptoxanthin, and an increase in β-carotene content. The content of β-carotene decreased significantly after the AcBCH1/2 genes were overexpressed in tomato. The content of zeaxanthin increased and β-carotene decreased in transgenic kiwifruit seedlings. The results will enrich our knowledge of the molecular mechanisms of carotenoid biosynthesis in kiwifruit.

SUBMITTER: Xia H

PROVIDER: S-EPMC9123235 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Characterization and functional validation of β-carotene hydroxylase AcBCH genes in Actinidia chinensis.

Xia Hui H Zhou Yuanjie Y Lin Zhiyi Z Guo Yuqi Y Liu Xinling X Wang Tong T Wang Jin J Deng Honghong H Lin Lijin L Deng Qunxian Q Lv Xiulan X Xu Kunfu K Liang Dong D

Horticulture research 20220314

Carotenoids are the pigment substances of yellow-fleshed kiwifruit, and among them β-cryptoxanthin has only been detected in the brighter yellow-fleshed variety 'Jinshi 1'. β-Carotene hydroxylase (BCH) catalyzes the formation of β-cryptoxanthin and zeaxanthin, but its molecular characteristics and functions have not been fully explained. Here we isolated two β-carotene hydroxylase genes, AcBCH1 and AcBCH2 from kiwifruit (Actinidia chinensis), and their relative expression le ...[more]

PMID: 35611182

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Project description:Traditional yellow maize though contains high kernel carotenoids, the concentration of provitamin A (proA) is quite low (<2 μg/g), compared to recommended level (15 μg/g). It also possesses poor endosperm protein quality due to low concentration of lysine and tryptophan. Natural variant of crtRB1 (β-carotene hydroxylase) and lcyE (lycopene-ε-cyclase) cause significant enhancement of proA concentration, while recessive allele, opaque2 (o2) enhances the level of these amino acids. Development of biofortified maize enriched in proA, lysine and tryptophan thus holds significance in alleviation of micronutrient malnutrition. In the present study, marker-assisted stacking of crtRB1, lcyE and o2 was undertaken in the genetic background of four maize hybrids (HQPM1, HQPM4, HQPM5, and HQPM7) popularly grown in India. HP704-22 and HP704-23 were used as donors, while four elite QPM parents viz., HKI161, HKI163, HKI193-1, and HKI193-2 were used as recipients. CrtRB1 showed severe segregation distortion, while lcyE segregated as per the expectation. Recovery of recurrent parent genome (RPG) among selected backcross progenies ranged from 89 to 93%. Introgressed progenies possessed high concentration of proA (7.38-13.59 μg/g), compared to 1.65-2.04 μg/g in the recurrent parents. The reconstituted hybrids showed an average of 4.5-fold increase in proA with a range of 9.25-12.88 μg/g, compared to original hybrids (2.14-2.48 μg/g). Similar plant-, ear-, and grain- characteristics of improved versions of both inbreds and hybrids were observed when evaluated with their respective original versions. Mean lysine (0.334%) and tryptophan (0.080%) of the improved hybrids were at par with the original versions (lysine: 0.340%, tryptophan: 0.083%). Improved hybrids also possessed similar grain yield potential (6,301-8,545 kg/ha) with their original versions (6,135-8,479 kg/ha) evaluated at two locations. This is the first study of staking crtRB1-, lcyE-, and o2-, favorable alleles in single genetic background. The improved inbreds can be effectively used as potential donor for independent and/or simultaneous introgression of crtRB1, lcyE, and o2 in the future breeding programme. These biofortified maize hybrids, rich in proA, lysine and tryptophan will hold great promise for nutritional security.

Dataset Information

Characterization and functional validation of β-carotene hydroxylase AcBCH genes in Actinidia chinensis.

Publications

Characterization and functional validation of β-carotene hydroxylase <i>AcBCH</i> genes in <i>Actinidia chinensis</i>.

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