Scan4CFU: Low-cost, open-source bacterial colony tracking over large areas and extended incubation times.

ABSTRACT: A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in exponential growth phase can be heterogeneous with respect to variables typically used to measure cell growth. Bacterial heterogeneity has important implications for how bacteria respond to environmental stresses, such as antibiotics. The phenomenon of antimicrobial persistence, for example, has been linked to a small subpopulation of cells that have entered into a state of dormancy where antibiotics are no longer effective. While methods have been developed for identifying individual non-growing cells in bacterial cultures, there has been less attenti

SUBMITTER: Pandey S 

PROVIDER: S-EPMC9123444 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature