PlateFlo - A software-controllable plate-scale perfusion system for culture of adherent cells.

ABSTRACT: Here we present a versatile system for milliliter-scale perfusion culture of adherent cells that can be built using basic tools, based on a readily available one-well culture plate (84 cm² culture area). Media composition and flow paths can be programmatically controlled via USB serial interface using the FETbox hardware controller and associated PlateFlo Python package. The FETbox can control up to five high current 12 V devices such as common pinch valves, solenoids, and DC motor peristaltic pumps. It was designed to be easily customized with built-in accommodation for additional electronic components (e.g. analog sensors and input), use of the ubiquitous Arduino Nano platform, and easily expanded serial communication protocol. Multiple FETboxes can be used in parallel for add

SUBMITTER: Pazdzior R 

PROVIDER: S-EPMC9123465 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature