Identification of TPBG-Expressing Amacrine Cells in DAT-tdTomato Mouse.

ABSTRACT:

Purpose

Neurons are the bricks of the neuronal system and experimental access to certain neuron subtypes will be of great help to decipher neuronal circuits. Here, we identified trophoblast glycoprotein (TPBG)-expressing GABAergic amacrine cells (ACs) that were selectively labeled in DAT-tdTomato transgenic mice.

Methods

Retina and brain sections were prepared for immunostaining with antibodies against various biomarkers. Patch-sequencing was performed to obtain the transcriptomes of tdTomato-positive cells in DAT-tdTomato mice. Whole-cell recordings were conducted to identify responses to light stimulation.

Results

Tyrosine hydroxylase immunoreactive cells were colocalized with tdTomato-positive cells in substantia nigra pars compacta, but not in the retina. Transcri

SUBMITTER: Huang W 

PROVIDER: S-EPMC9123489 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature