Programmable protein topology via SpyCatcher-SpyTag chemistry in one-pot cell-free expression system.

ABSTRACT: Protein-based biomaterials play a significant role in biomedicine and biocatalysis due to their intrinsic biocompatibility and biodegradability. Topological biomaterials show certain advantages without changing the wild-type sequence of the protein, such as unique biofunctions and exceptional stabilities. However, the tuning for the synthesis and assembly of topological protein materials was limited. In this study, we combined the SpyCatcher/SpyTag (SC/ST) chemistry and proposed a cell-free one-pot transcription-translation-assembly system for flexibly regulating the production of topological protein materials. Dimers, trimers, and multimers of proteins with topological structures were designed. Next, the cell-free system was optimized by adjusting the magnesium ion concentration and the m

SUBMITTER: Ye Q 

PROVIDER: S-EPMC9123646 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature