METTL16 promotes hepatocellular carcinoma progression through downregulating RAB11B-AS1 in an m⁶A-dependent manner.

ABSTRACT:

Background

The molecular mechanisms driving hepatocellular carcinoma (HCC) remain largely unclear. As one of the major epitranscriptomic modifications, N⁶-methyladenosine (m⁶A) plays key roles in HCC. The aim of this study was to investigate the expression, roles, and mechanisms of action of the RNA methyltransferase methyltransferase-like protein 16 (METTL16) in HCC.

Methods

The expression of METTL16 and RAB11B-AS1 was determined by RT-qPCR. The regulation of RAB11B-AS1 by METTL16 was investigated by RNA immunoprecipitation (RIP), methylated RIP (MeRIP), and RNA stability assays. In vitro and in vivo gain- and loss-of-function assays were performed to investigate the roles of METTL16 and RAB11B-AS1.

Results

METTL16 was upregulated in HCC, and it

SUBMITTER: Dai YZ 

PROVIDER: S-EPMC9123709 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature