Dataset Information
Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii.
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ABSTRACT: Objective
Toxoplasma gondii is a ubiquitous parasite of medical and veterinary importance; however, there exists no cure for chronic toxoplasmosis. Metabolic enzymes required for the production and maintenance of tissue cysts represent promising targets for novel therapies. Here, we use reverse genetics to investigate the role of Toxoplasma phosphoglucomutase 1, PGM1, in Toxoplasma growth and cystogenesis.Results
We found that disruption of pgm1 did not significantly affect Toxoplasma intracellular growth and the lytic cycle. pgm1-defective parasites could differentiate into bradyzoites and produced cysts containing amylopectin in vitro. However, cysts produced in the absence of pgm1 were significantly smaller than wildtype. Together, our findings suggest that PGM1 is dispensable for in vitro growth but contributes to optimal Toxoplasma cyst development in vitro, thereby necessitating further investigation into the function of this enzyme in Toxoplasma persistence in its host.
SUBMITTER: Quach EV
PROVIDER: S-EPMC9123713 | biostudies-literature | 2022 May
REPOSITORIES: biostudies-literature
Publications
Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii.
BMC research notes 20220521 1
<h4>Objective</h4>Toxoplasma gondii is a ubiquitous parasite of medical and veterinary importance; however, there exists no cure for chronic toxoplasmosis. Metabolic enzymes required for the production and maintenance of tissue cysts represent promising targets for novel therapies. Here, we use reverse genetics to investigate the role of Toxoplasma phosphoglucomutase 1, PGM1, in Toxoplasma growth and cystogenesis.<h4>Results</h4>We found that disruption of pgm1 did not significantly affect Toxop ...[more]