StaRTrEK:in silico estimation of RNA half-lives from genome-wide time-course experiments without transcriptional inhibition.

ABSTRACT:

Background

Gene expression is the result of the balance between transcription and degradation. Recent experimental findings have shown fine and specific regulation of RNA degradation and the presence of various molecular machinery purposely devoted to this task, such as RNA binding proteins, non-coding RNAs, etc. A biological process can be studied by measuring time-courses of RNA abundance in response of internal and/or external stimuli, using recent technologies, such as the microarrays or the Next Generation Sequencing devices. Unfortunately, the picture provided by looking only at the transcriptome abundance may not gain insight into its dynamic regulation. By contrast, independent simultaneous measurement of RNA expression and half-lives could provide such valuable additional

SUBMITTER: Conte F 

PROVIDER: S-EPMC9123730 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature