Proteomic characterization of post-mortem human brain tissue following ultracentrifugation-based subcellular fractionation.

ABSTRACT: Proteomic characterization of human brain tissue is increasingly utilized to identify potential novel biomarkers and drug targets for a variety of neurological diseases. In whole-tissue studies, results may be driven by changes in the proportion of the largest and most abundant organelles or tissue cell-type composition. Spatial proteomics approaches enhance our knowledge of disease mechanisms and changing signalling pathways at the subcellular level by taking into account the importance of cellular localization, which critically influences protein function. Density gradient-based ultracentrifugation methods allow for subcellular fractionation and have been utilized in cell lines, mouse and human brain tissue to quantify thousands of proteins in specific enriched organelles such as the pre

SUBMITTER: Kandigian SE 

PROVIDER: S-EPMC9123841 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature