Identification and Characterization of Antigen-Specific CD8⁺ T Cells Using Surface-Trapped TNF-α and Single-Cell Sequencing.

ABSTRACT: CD8⁺ T cells are key mediators of antiviral and antitumor immunity. The isolation and study of Ag-specific CD8⁺ T cells, as well as mapping of their MHC restriction, has practical importance to the study of disease and the development of therapeutics. Unfortunately, most experimental approaches are cumbersome, owing to the highly variable and donor-specific nature of MHC-bound peptide/TCR interactions. Here we present a novel system for rapid identification and characterization of Ag-specific CD8⁺ T cells, particularly well suited for samples with limited primary cells. Cells are stimulated ex vivo with Ag of interest, followed by live cell sorting based on surface-trapped TNF-α. We take advantage of major advances in single-cell sequencing to generate full

SUBMITTER: Abdulhaqq S 

PROVIDER: S-EPMC9124229 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature