The association between Annexin A2 and epithelial cell adhesion molecule in breast cancer cells.

ABSTRACT:

Background

The epithelial cell adhesion molecule (EpCAM) is a type I transmembrane and glycosylated protein, which is overexpressed in many neoplasms. However, EpCAM has no known ligand partners and the mechanisms by which it functions are not fully understood.

Aim

This study was performed to discover novel partners of EpCAM, which may provide a better understanding of its functions.

Methods

The membrane fraction of the ERα⁺ noninvasive breast cancer cell line ZR-75-1 and MCF-7 was extracted and followed by co-immunoprecipitation of EpCAM using C-10, a mouse monoclonal antibody raised against amino acids 24-93 of the EpCAM molecule. As a negative control, MDA-MB-231 and Hs578T were used since they express a negligible amount of EpCAM and are known as EpCAM<

SUBMITTER: Al-Qahtani SM 

PROVIDER: S-EPMC9124509 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature