Derivation of extra-embryonic and intra-embryonic macrophage lineages from human pluripotent stem cells.

ABSTRACT: Tissue-resident macrophages are increasingly recognized as important determinants of organ homeostasis, tissue repair, remodeling and regeneration. Although the ontogeny and function of tissue-resident macrophages has been identified as distinct from postnatal hematopoiesis, the inability to specify, in vitro, similar populations that recapitulate these developmental waves has limited our ability to study their function and potential for regenerative applications. We took advantage of the concept that tissue-resident macrophages and monocyte-derived macrophages originate from distinct extra-embryonic and definitive hematopoietic lineages to devise a system to generate pure cultures of macrophages that resemble tissue-resident or monocyte-derived subsets. We demonstrate that human pluripote

SUBMITTER: Bredemeyer AL 

PROVIDER: S-EPMC9124573 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature