Influence of SARS-CoV-2 inactivation by different chemical reagents on the humoral response evaluated in a murine model.

ABSTRACT: Viral inactivation for antibody induction purposes, among other applications, should ensure biosafety, completely avoiding the risk of infectivity, and preserving viral immunogenicity. β-propiolactone (BPL) is one of the most used reagents for viral inactivation, despite its high toxicity and recent difficulties related to importation, experienced in Brazil during the SARS-CoV-2 pandemic. In this context, the main objectives of this work were to test different inactivation procedures for SARS-CoV-2 and to evaluate the induction of neutralizing antibodies in mice immunized with antigenic preparations obtained after viral treatment with formaldehyde (FDE), glutaraldehyde (GDE), peroxide hydrogen (H₂O₂), as well as with viral proteins extract (VPE), in parallel with BPL.

SUBMITTER: de Castro Barbosa E 

PROVIDER: S-EPMC9125173 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature