A Porcine Sepsis Model With Numerical Scoring for Early Prediction of Severity.

ABSTRACT:

Introduction

Sepsis can lead to organ dysfunctions with disturbed oxygen dynamics and life-threatening consequences. Since the results of organ-protective treatments cannot always be transferred from laboratory models into human therapies, increasing the translational potential of preclinical settings is an important goal. Our aim was to develop a standardized research protocol, where the progression of sepsis-related events can be characterized reproducibly in model experiments within clinically-relevant time frames.

Methods

Peritonitis was induced in anesthetized minipigs injected intraperitoneally with autofeces inoculum (n = 27) or with saline (sham operation; n = 9). The microbial colony-forming units (CFUs) in the inoculum were retrospectively determined.

SUBMITTER: Rutai A 

PROVIDER: S-EPMC9125192 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature