Challenges in the use of sortase and other peptide ligases for site-specific protein modification.

ABSTRACT: Site-specific protein modification is a widely-used biochemical tool. However, there are many challenges associated with the development of protein modification techniques, in particular, achieving site-specificity, reaction efficiency and versatility. The engineering of peptide ligases and their substrates has been used to address these challenges. This review will focus on sortase, peptidyl asparaginyl ligases (PALs) and variants of subtilisin; detailing how their inherent specificity has been utilised for site-specific protein modification. The review will explore how the engineering of these enzymes and substrates has led to increased reaction efficiency mainly due to enhanced catalytic activity and reduction of reversibility. It will also describe how engineering peptide ligases to br

SUBMITTER: Morgan HE 

PROVIDER: S-EPMC9126251 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature