Evaluating a targeted multiple reaction monitoring approach to global untargeted lipidomic analyses of human plasma.

ABSTRACT:

Rationale

The Lipidyzer platform was recently updated on a SCIEX QTRAP 6500+ mass spectrometer and offers a targeted lipidomics assay including 1150 different lipids. We evaluated this targeted approach using human plasma samples and compared the results against a global untargeted lipidomics method using a high-resolution Q Exactive HF Orbitrap mass spectrometer.

Methods

Lipids from human plasma samples (N = 5) were extracted using a modified Bligh-Dyer approach. A global untargeted analysis was performed using a Thermo Orbitrap Q Exactive HF mass spectrometer, followed by data analysis using Progenesis QI software. Multiple reaction monitoring (MRM)-based targeted analysis was performed using a QTRAP 6500+ mass spectrometer, followed by data analysis using SCIEX OS softwar

SUBMITTER: Khan MJ 

PROVIDER: S-EPMC9126483 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature