Contractile force assessment methods for in vitro skeletal muscle tissues.

ABSTRACT: Over the last few years, there has been growing interest in measuring the contractile force (CF) of engineered muscle tissues to evaluate their functionality. However, there are still no standards available for selecting the most suitable experimental platform, measuring system, culture protocol, or stimulation patterns. Consequently, the high variability of published data hinders any comparison between different studies. We have identified that cantilever deflection, post deflection, and force transducers are the most commonly used configurations for CF assessment in 2D and 3D models. Additionally, we have discussed the most relevant emerging technologies that would greatly complement CF evaluation with intracellular and localized analysis. This review provides a comprehensive analysis of

SUBMITTER: Vesga-Castro C 

PROVIDER: S-EPMC9126583 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature