Enhanced detection sensitivity through enzyme-induced precipitate accumulation in LSPR-active nano-valleys.

ABSTRACT: Biomolecule detection based on the localized surface plasmon resonance (LSPR) phenomenon has advantages in label-free detection, good sensitivity, and measurement simplicity and reproducibility. However, in order to ultimately be used for actual diagnosis, the ability to detect trace amounts of biomarkers is necessary, which requires the development of signal enhancement strategies that enable ultrasensitive detection. In this paper, we provide a straightforward and efficient route to boost LSPR sensitivity based on multiple sample washings. We found that repeated washing and drying cycles lead to a shift in the LSPR peak in a concentration-dependent manner, where this process drives the accumulation of a precipitate, formed by an enzyme reaction with target specificity, in the sample's LS

SUBMITTER: Kwak SH 

PROVIDER: S-EPMC9126672 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature