Identification of nontuberculous mycobacteria isolated from household showerheads of patients with nontuberculous mycobacteria.

ABSTRACT: This study aimed to examine whether nontuberculous mycobacteria (NTM) inside household showerheads are identical to those in patients with NTM-pulmonary disease (PD) since household water is one of the potential NTM sources. Samples were obtained from 32 household showerheads of patients with NTM-PD recruited through the Pulmonary Outpatient Department at the Severance Hospital between October 2018 and October 2019. All isolates from patients with NTM-PD were diagnosed using a reverse-hybridization line probe assay based on the ropB gene. To determine the mycobacterial compositions, the washing fluids were collected and investigated using multiplex polymerase chain reaction assay and NTM culture; suspected microbial isolates in these fluids and culture were identified using sequencing anal

SUBMITTER: Choi JY 

PROVIDER: S-EPMC9127090 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature