Single-cell RNA sequencing unravels heterogeneity of skeletal progenitors and cell-cell interactions underlying the bone repair process.

ABSTRACT:

Introduction

Activation of skeletal progenitors upon tissue injury and the subsequent cell fate specification are tightly coordinated in the bone repair process. Although known osteoimmunological signaling networks play important roles in the microenvironment of the bone defect sites, the molecular mechanism underlying the bone repair process has not been fully understood.

Methods

To better understand the behavior of the skeletal progenitors and the heterogeneity of the cells during bone repair at the microenvironmental level, we performed a combinatorial analysis consisting of lineage tracing for skeletal progenitors using the Sox9-CreERT2;R26R ^tdTomato mouse line followed by single-cell RNA sequencing (scRNA-seq) analysis using a mouse model of cal

SUBMITTER: Nakayama M 

PROVIDER: S-EPMC9127115 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature