αα-hub coregulator structure and flexibility determine transcription factor binding and selection in regulatory interactomes.

ABSTRACT: Formation of transcription factor (TF)-coregulator complexes is a key step in transcriptional regulation, with coregulators having essential functions as hub nodes in molecular networks. How specificity and selectivity are maintained in these nodes remain open questions. In this work, we addressed specificity in transcriptional networks using complexes formed between TFs and αα-hubs, which are defined by a common αα-hairpin secondary structure motif, as a model. Using NMR spectroscopy and binding thermodynamics, we analyzed the structure, dynamics, stability, and ligand-binding properties of the Arabidopsis thaliana RST domains from TAF4 and known binding partner RCD1, and the TAFH domain from human TAF4, allowing comparison across species, functions, and architectural contexts. While thes

SUBMITTER: Friis Theisen F 

PROVIDER: S-EPMC9127584 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature