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A modified surgical procedure using minimally invasive ileocolic vein perfusion in a mouse intrahepatic islet transplant model.

ABSTRACT: Murine intrahepatic islet transplantation is a clinically relevant but technically challenging surgical procedure because of frequent lethal postoperative bleeding. Here, we describe a protocol for mouse pancreatic islet isolation, purification, and culture. Besides, we also describe a protocol for intrahepatic islet transplantation through the ileocolic vein. Intrahepatic islet transplantation through the ileocolic vein, as opposed to traditional islet perfusion via the main portal vein, has the advantage of improving recovery after surgery and may facilitate islet survival and function in preclinical settings. For complete details on the use and execution of this protocol, please refer to Shrestha et al. (2020).

SUBMITTER: Zhang L

PROVIDER: S-EPMC9127695 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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A modified surgical procedure using minimally invasive ileocolic vein perfusion in a mouse intrahepatic islet transplant model.

Zhang Lei L Turan Ali A Shirwan Haval H Yolcu Esma S ES

STAR protocols 20220519 2

Murine intrahepatic islet transplantation is a clinically relevant but technically challenging surgical procedure because of frequent lethal postoperative bleeding. Here, we describe a protocol for mouse pancreatic islet isolation, purification, and culture. Besides, we also describe a protocol for intrahepatic islet transplantation through the ileocolic vein. Intrahepatic islet transplantation through the ileocolic vein, as opposed to traditional islet perfusion via the main portal vein, has th ...[more]

PMID: 35620067

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