Structure of Geobacter pili reveals secretory rather than nanowire behaviour.

ABSTRACT: Extracellular electron transfer by Geobacter species through surface appendages known as microbial nanowires¹ is important in a range of globally important environmental phenomena², as well as for applications in bio-remediation, bioenergy, biofuels and bioelectronics. Since 2005, these nanowires have been thought to be type 4 pili composed solely of the PilA-N protein¹. However, previous structural analyses have demonstrated that, during extracellular electron transfer, cells do not produce pili but rather nanowires made up of the cytochromes OmcS^2,3 and OmcZ⁴. Here we show that Geobacter sulfurreducens binds PilA-N to PilA-C to assemble heterodimeric pili, which remain periplasmic under nanowire-producing conditions that require extr

SUBMITTER: Gu Y 

PROVIDER: S-EPMC9127704 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature