Comparative Analysis of Metagenomics and Metataxonomics for the Characterization of Vermicompost Microbiomes.

ABSTRACT: The study of microbial communities or microbiotas in animals and environments is important because of their impact in a broad range of industrial applications, diseases and ecological roles. High throughput sequencing (HTS) is the best strategy to characterize microbial composition and function. Microbial profiles can be obtained either by shotgun sequencing of genomes, or through amplicon sequencing of target genes (e.g., 16S rRNA for bacteria and ITS for fungi). Here, we compared both HTS approaches at assessing taxonomic and functional diversity of bacterial and fungal communities during vermicomposting of white grape marc. We applied specific HTS workflows to the same 12 microcosms, with and without earthworms, sampled at two distinct phases of the vermicomposting process occurring at

SUBMITTER: Perez-Losada M 

PROVIDER: S-EPMC9127802 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature