Rapid and Accurate Identification of SARS-CoV-2 Variants Using Real Time PCR Assays.

ABSTRACT:

Background

Genomic surveillance efforts for SARS-CoV-2 are needed to understand the epidemiology of the COVID-19 pandemic. Viral variants may impact routine diagnostic testing, increase viral transmissibility, cause differences in disease severity, have decreased susceptibility to therapeutics, and/or confer the ability to evade host immunity. While viral whole-genome sequencing (WGS) has played a leading role in surveillance programs, many laboratories lack the expertise and resources for performing WGS. This study describes the performance of multiplexed real-time reverse transcription-PCR (RT-PCR) assays for identification of SARS-CoV-2 variants.

Methods

SARS-CoV-2 specimens were tested for spike-gene variants using a combination of allele-specific primer and allele-speci

SUBMITTER: Borillo GA 

PROVIDER: S-EPMC9127862 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature