Synthetic Translational Regulation by Protein-Binding RNA Origami Scaffolds.

ABSTRACT: Rational design approaches for the regulation of gene expression are expanding the synthetic biology toolbox. However, only a few tools for regulating gene expression at the translational level have been developed. Here, we devise an approach for translational regulation using the MS2 and PP7 aptamer and coat-protein pairs in Escherichia coli. The aptamers are used as operators in transcription units that encode proteins fused to their cognate coat proteins, which leads to self-repression. RNA origami scaffolds that contain up to four aptamers serve as an alternate binder to activate translation. With this system, we demonstrate that the increase in expression of a reporter protein is dependent on both the concentration and number of aptamers on RNA origami scaffolds. We also demons

SUBMITTER: Nguyen MTA 

PROVIDER: S-EPMC9127956 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature