Longitudinal profiling of clonal hematopoiesis provides insight into clonal dynamics.

ABSTRACT:

Background

Clonal hematopoiesis of indeterminate potential (CHIP), the age-related expansion of mutant hematopoietic stem cells, confers risk for multiple diseases of aging including hematologic cancer and cardiovascular disease. Whole-exome or genome sequencing can detect CHIP, but due to those assays' high cost, most population studies have been cross-sectional, sequencing only a single timepoint per individual.

Results

We developed and validated a cost-effective single molecule molecular inversion probe sequencing (smMIPS) assay for detecting CHIP, targeting the 11 most frequently mutated genes in CHIP along with 4 recurrent mutational hotspots. We sequenced 548 multi-timepoint samples collected from 182 participants in the Women's Health Initiative cohort, across a media

SUBMITTER: Uddin MM 

PROVIDER: S-EPMC9128083 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature