Cloning and functional verification of a porcine adipose tissue-specific promoter.

ABSTRACT:

Background

Fat deposition is an important economic trait in pigs. In the past decades, many genes regulating porcine fat deposition were identified by Omics technology and verified by cell biology studies. Using genetically modified pigs to investigate the function of these genes in vivo is necessary before applying in breeding. However, lack of tissue-specific promoters of pigs hinders the generation of adipose tissue-specific genetically modified pigs.

Results

In order to identify a porcine adipose tissue-specific promoter, we used the software Digital Differential Display (DDD) to screen 99 genes highly expressed in porcine adipose tissue. GO and KEGG enrichment analysis indicated that the 99 genes were mainly related to lipid metabolism. Q-PCR proved that LGALS12 was an

SUBMITTER: Zhang D 

PROVIDER: S-EPMC9128115 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature