The link between menin and pleiotrophin in the tumor biology of pancreatic neuroendocrine neoplasms.

ABSTRACT: MEN1, which encodes menin protein, is the most frequently mutated gene in pancreatic neuroendocrine neoplasms (pNEN). Pleiotrophin (PTN) has been reported as a downstream factor of menin that promotes metastasis in different tumor entities. In this study, the effect of menin and its link to PTN were assessed using features of pNEN cells and the outcome of patients with pNEN. The expression levels of menin and PTN in tissues from patients with pNEN were examined using qRT-PCR and western blot and compared with their metastasis status. Functional assays, including transwell migration/invasion and scratch wound-healing assays, were performed on specifically designed CRISPR/Cas9-mediated MEN1-knockout (MEN1-KO) pNEN cell lines (BON1^MEN1-KO and QGP1^MEN1-KO ) to study the m

SUBMITTER: He L 

PROVIDER: S-EPMC9128182 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature