Lab-on-chip assay of tumour markers and human papilloma virus for cervical cancer detection at the point-of-care.

ABSTRACT: Cervical cancer affects over half a million people worldwide each year, the majority of whom are in resource-limited settings where cytology screening is not available. As persistent human papilloma virus (HPV) infections are a key causative factor, detection of HPV strains now complements cytology where screening services exist. This work demonstrates the efficacy of a handheld Lab-on-Chip (LoC) device, with an external sample extraction process, in detecting cervical cancer from biopsy samples. The device is based on Ion-Sensitive Field-Effect Transistor (ISFET) sensors used in combination with loop-mediated isothermal amplification (LAMP) assays, to amplify HPV DNA and human telomerase reverse transcriptase (hTERT) mRNA. These markers were selected because of their high levels of expres

SUBMITTER: Wormald BW 

PROVIDER: S-EPMC9128326 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature