Subnuclear localisation is associated with gene expression more than parental origin at the imprinted Dlk1-Dio3 locus.

ABSTRACT: At interphase, de-condensed chromosomes have a non-random three-dimensional architecture within the nucleus, however, little is known about the extent to which nuclear organisation might influence expression or vice versa. Here, using imprinting as a model, we use 3D RNA- and DNA-fluorescence-in-situ-hybridisation in normal and mutant mouse embryonic stem cell lines to assess the relationship between imprinting control, gene expression and allelic distance from the nuclear periphery. We compared the two parentally inherited imprinted domains at the Dlk1-Dio3 domain and find a small but reproducible trend for the maternally inherited domain to be further away from the periphery however we did not observe an enrichment of inactive alleles in the immediate vicinity of the nuclear envelope. Us

SUBMITTER: Mashoodh R 

PROVIDER: S-EPMC9129038 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature