Structural dynamics of SARS-CoV-2 nucleocapsid protein induced by RNA binding.

ABSTRACT: The nucleocapsid (N) protein of the SARS-CoV-2 virus, the causal agent of COVID-19, is a multifunction phosphoprotein that plays critical roles in the virus life cycle, including transcription and packaging of the viral RNA. To play such diverse roles, the N protein has two globular RNA-binding modules, the N- (NTD) and C-terminal (CTD) domains, which are connected by an intrinsically disordered region. Despite the wealth of structural data available for the isolated NTD and CTD, how these domains are arranged in the full-length protein and how the oligomerization of N influences its RNA-binding activity remains largely unclear. Herein, using experimental data from electron microscopy and biochemical/biophysical techniques combined with molecular modeling and molecular dynamics simulations

SUBMITTER: Ribeiro-Filho HV 

PROVIDER: S-EPMC9129039 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature