HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies.

ABSTRACT: African swine fever (ASF), which is caused by the ASF virus (ASFV), is a highly contagious hemorrhagic disease that causes high mortality to domestic porcine and wild boars and brings huge economic losses to world swine industry. Due to the lack of an effective vaccine, the control of ASF must depend on early, efficient, and cost-effective detection and strict control and elimination strategies. Traditional serological testing methods are generally associated with high testing costs, complex operations, and high technical requirements. As a promising alternative diagnostic tool to traditional antibodies, nanobodies (Nb) have the advantages of simpler and faster generation, good stability and solubility, and high affinity and specificity, although the system is dependent on the immunization

SUBMITTER: Zhao H 

PROVIDER: S-EPMC9130055 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature