Detection of ¹³C labeling of glutamate and glutamine in human brain by proton magnetic resonance spectroscopy.

ABSTRACT: A proton magnetic resonance spectroscopy (MRS) technique was used to measure ¹³C enrichments of glutamate and glutamine in a 3.5 × 1.8 × 2 cm³ voxel placed in the dorsal anterior cingulate cortex of five healthy participants after oral administration of [U-¹³C]glucose. Strong pseudo singlets of glutamate and glutamine were induced to enhance the signal strength of glutamate and glutamine. This study demonstrated that ¹³C labeling of glutamate and glutamine can be measured with the high sensitivity and spatial resolution of ¹H MRS using a proton-only MRS technique with standard commercial hardware. Furthermore, it is feasible to measure ¹³C labeling of glutamate and glutamine in limbic structures, which play major roles in be

SUBMITTER: An L 

PROVIDER: S-EPMC9130156 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature