Ectopic expression of Vigna radiata's vacuolar Na⁺/H⁺ antiporter gene (VrNHX1) in indica rice (Oryza sativa L.).

ABSTRACT: It is essential to develop high salt-tolerant rice varieties in order to cultivate the salt-affected lands. In this study, Na⁺/H⁺ exchanger 1 (NHX1) gene isolated from Vigna radiata L. Wilczek was transferred in Bangladesh Rice Research Institute (BRRI) developed two indica rice genotypes BRRI Dhan28 and BRRI Dhan29 using in-planta approach for improvement of salinity tolerance. Embryonic axes of matured dehusked rice seeds were injured and co-cultivated with Agrobacterium strain harboring VrNHX1 gene and finally regenerated. GUS histochemical assay and PCR amplification of GUS-a and VrNHX1 were performed to confirm the transformation. Expression confirmation was done by semi-quantitative RT-PCR. Under salinity stress, transgenic lines show

SUBMITTER: Hasan MN 

PROVIDER: S-EPMC9130519 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature