Yeast cells depleted of the frataxin homolog Yfh1 redistribute cellular iron: Studies using Mossbauer spectroscopy and mathematical modeling.

ABSTRACT: The neurodegenerative disease Friedreich's ataxia arises from a deficiency of frataxin, a protein that promotes iron-sulfur cluster (ISC) assembly in mitochondria. Here, primarily using Mössbauer spectroscopy, we investigated the iron content of a yeast strain in which expression of yeast frataxin homolog 1 (Yfh1), oxygenation conditions, iron concentrations, and metabolic modes were varied. We found that aerobic fermenting Yfh1-depleted cells grew slowly and accumulated Fe^III nanoparticles, unlike WT cells. Under hypoxic conditions, the same mutant cells grew at rates similar to WT cells, had similar iron content, and were dominated by Fe^II rather than Fe^III nanoparticles. Furthermore, mitochondria from mutant hypoxic cells contained approximately the same

SUBMITTER: Fernandez S 

PROVIDER: S-EPMC9130540 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature