The Alternative Sigma Factor SigL Influences <i>Clostridioides difficile</i> Toxin Production, Sporulation, and Cell Surface Properties.

ABSTRACT: The alternative sigma factor SigL (Sigma-54) facilitates bacterial adaptation to the extracellular environment by modulating the expression of defined gene subsets. A homolog of the gene encoding SigL is conserved in the diarrheagenic pathogen Clostridioides difficile. To explore the contribution of SigL to C. difficile biology, we generated sigL-disruption mutants (sigL::erm) in strains belonging to two phylogenetically distinct lineages-the human-relevant Ribotype 027 (strain BI-1) and the veterinary-relevant Ribotype 078 (strain CDC1). Comparative proteomics analyses of mutants and isogenic parental strains revealed lineage-specific SigL regulons. Concomitantly, loss of SigL resulted in pleiotropic and distinct phenotypic alterations in the two strains. Sporu

SUBMITTER: Clark AE 

PROVIDER: S-EPMC9130780 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature