Single-cell analysis reveals innate lymphoid cell lineage infidelity in atopic dermatitis.

ABSTRACT:

Background

Although ample knowledge exists about phenotype and function of cutaneous T lymphocytes, much less is known about the lymphocytic components of the skin's innate immune system.

Objective

To better understand the biologic role of cutaneous innate lymphoid cells (ILCs), we investigated their phenotypic and molecular features under physiologic (normal human skin [NHS]) and pathologic (lesional skin of patients with atopic dermatitis [AD]) conditions.

Methods

Skin punch biopsies and reduction sheets as well as blood specimens were obtained from either patients with AD or healthy individuals. Cell and/or tissue samples were analyzed by flow cytometry, immunohistochemistry, and single-cell RNA sequencing or subjected to in vitro/ex vivo culture.

Results

No

SUBMITTER: Alkon N 

PROVIDER: S-EPMC9130781 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature