Cytosolic Nudix Hydrolase 1 Is Involved in Geranyl β-Primeveroside Production in Tea.

ABSTRACT: Geraniol is a potent tea odorant and exists mainly as geranyl glycoside in Camellia sinensis. Understanding the mechanisms of geraniol biosynthesis at molecular levels in tea plants is of great importance for practical improvement of tea aroma. In this study, geraniol and its glycosides from tea plants were examined using liquid chromatography coupled with mass spectrometry. Two candidate geraniol synthase (GES) genes (CsTPS) and two Nudix hydrolase genes (CsNUDX1-cyto and CsNUDX1-chlo) from the tea genome were functionally investigated through gene transcription manipulation and gene chemical product analyses. Our data showed that in tea leaves, levels of geranyl β-primeveroside were dramatically higher than those of geranyl β-glucoside, wh

SUBMITTER: Zhou H 

PROVIDER: S-EPMC9131077 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature