Supplementation with omega-3 fatty acids potentiates oxidative stress in human airway epithelial cells exposed to ozone.

ABSTRACT:

Background

Dietary intake of the omega-3 family of polyunsaturated fatty acids (ω-3 FA) is associated with anti-inflammatory effects. However, unsaturated fatty acids are susceptible to oxidation, which produces pro-inflammatory mediators. Ozone (O₃) is a tropospheric pollutant that reacts rapidly with unsaturated fatty acids to produce electrophilic and oxidative mediators of inflammation.

Objective

Determine whether supplementation with ω-3 FA alters O₃-induced oxidative stress in human airway epithelial cells (HAEC).

Methods

16-HBE cells expressing a genetically encoded sensor of the reduced to oxidized glutathione ratio (GSH/GSSG, E_GSH) were supplemented with saturated, monounsaturated, or ω-3 FA prior to exposure to 0, 0.08, 0.1,

SUBMITTER: Corteselli EM 

PROVIDER: S-EPMC9131300 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature