Sabatier Principle for Rationalizing Enzymatic Hydrolysis of a Synthetic Polyester.

ABSTRACT: Interfacial enzyme reactions are common in Nature and in industrial settings, including the enzymatic deconstruction of poly(ethylene terephthalate) (PET) waste. Kinetic descriptions of PET hydrolases are necessary for both comparative analyses, discussions of structure-function relations and rational optimization of technical processes. We investigated whether the Sabatier principle could be used for this purpose. Specifically, we compared the kinetics of two well-known PET hydrolases, leaf-branch compost cutinase (LCC) and a cutinase from the bacterium Thermobifida fusca (TfC), when adding different concentrations of the surfactant cetyltrimethylammonium bromide (CTAB). We found that CTAB consistently lowered the strength of enzyme-PET interactions, while its effect on enzymatic t

SUBMITTER: Arnling Baath J 

PROVIDER: S-EPMC9131473 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature