Exogenous leptin enhances markers of airway fibrosis in a mouse model of chronic allergic airways disease.

ABSTRACT:

Background

Asthma patients with comorbid obesity exhibit increased disease severity, in part, due to airway remodeling, which is also observed in mouse models of asthma and obesity. A mediator of remodeling that is increased in obesity is leptin. We hypothesized that in a mouse model of allergic airways disease, mice receiving exogenous leptin would display increased airway inflammation and fibrosis.

Methods

Five-week-old male and female C57BL/6J mice were challenged with intranasal house dust mite (HDM) allergen or saline 5 days per week for 6 weeks (n = 6-9 per sex, per group). Following each HDM exposure, mice received subcutaneous recombinant human leptin or saline. At 48 h after the final HDM challenge, lung mechanics were evaluated and the mice were sacrificed. Broncho

SUBMITTER: Ihrie MD 

PROVIDER: S-EPMC9131622 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature