Metformin exerts an antitumor effect by inhibiting bladder cancer cell migration and growth, and promoting apoptosis through the PI3K/AKT/mTOR pathway.

ABSTRACT:

Background

To observe and explore the effect of metformin on the migration and proliferation of bladder cancer T24 and 5637 cells in vitro.

Methods

Bladder cancer T24 and 5637 cell lines were cultured in vitro, and were divided into group A (blank control group) and group B (metformin group: 5, 10, 15, and 20 mmol/L); both groups were plated on 6-well plates at the same time. Culture in 24-well plates was used for wound healing assays and in 96-well plates for Transwell migration and invasion, and Cell Counting Kit-8 proliferation experiments. We observed and detected the cell migration and proliferation ability of each group at 48 h, and calculated the cell migration area and survival rate. Flow cytometry was used to detect cell apoptosis in the groups. The apoptosis-relate

SUBMITTER: Shen Z 

PROVIDER: S-EPMC9131696 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature