K⁺ efflux through postsynaptic NMDA receptors suppresses local astrocytic glutamate uptake.

ABSTRACT: Glutamatergic transmission prompts K⁺ efflux through postsynaptic NMDA receptors. The ensuing hotspot of extracellular K⁺ elevation depolarizes presynaptic terminal, boosting glutamate release, but whether this also affects glutamate uptake in local astroglia has remained an intriguing question. Here, we find that the pharmacological blockade, or conditional knockout, of postsynaptic NMDA receptors suppresses use-dependent increase in the amplitude and duration of the astrocytic glutamate transporter current (I_GluT ), whereas blocking astrocytic K⁺ channels prevents the duration increase only. Glutamate spot-uncaging reveals that astrocyte depolarization, rather than extracellular K⁺ rises per se, is required to reduce the amplitude an

SUBMITTER: Tyurikova O 

PROVIDER: S-EPMC9132042 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature