The Effects of Propofol on a Human in vitro Blood-Brain Barrier Model.

ABSTRACT:

Background

Recently, the safety of repeated and lengthy anesthesia administration has been called into question, a subset of these animal studies demonstrated that anesthetics induced blood-brain barrier (BBB) dysfunction. The BBB is critical in protecting the brain parenchyma from the surrounding micro-vasculature. BBB breakdown and dysfunction has been observed in several neurodegenerative diseases and may contribute to both the initiation and the progression of the disease. In this study we utilize a human induced pluripotent stem cell (iPSC) derived-BBB model, exhibiting near in vivo properties, to evaluate the effects of anesthetics on critical barrier properties.

Methods

iPSC-derived brain microvascular endothelial cells (BMECs) expressed near in vivo bar

SUBMITTER: Hughes JM 

PROVIDER: S-EPMC9132176 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature