PEGDA microencapsulated allogeneic islets reverse canine diabetes without immunosuppression.

ABSTRACT:

Background

Protection of islets without systemic immunosuppression has been a long-sought goal in the islet transplant field. We conducted a pilot biocompatibility/safety study in healthy dogs followed by a dose-finding efficacy study in diabetic dogs using polyethylene glycol diacrylate (PEGDA) microencapsulated allogeneic canine islets.

Methods

Prior to the transplants, characterization of the canine islets included the calculations determining the average cell number/islet equivalent. Following measurements of purity, insulin secretion, and insulin, DNA and ATP content, the islets were encapsulated and transplanted interperitoneally into dogs via a catheter, which predominantly attached to the omentum. In the healthy dogs, half of the microspheres injected contained canin

SUBMITTER: Harrington S 

PROVIDER: S-EPMC9132281 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature