Quantifying telomeric lncRNAs using PNA-labelled RNA-Flow FISH (RNA-Flow).
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ABSTRACT:
SUBMITTER: Gonzalez-Vasconcellos I
PROVIDER: S-EPMC9132901 | biostudies-literature | 2022 May
REPOSITORIES: biostudies-literature
Publications
Quantifying telomeric lncRNAs using PNA-labelled RNA-Flow FISH (RNA-Flow).
González-Vasconcellos Iria I Cobos-Fernández María A MA Atkinson Michael J MJ Fernandez-Piqueras José J Santos Javier J
Communications biology 20220525 1
Here we present a method to detect and quantify long non-coding RNAs, in particular those related to telomeres. By coupling the specificity of a peptide nucleic acid (PNA) probe with flow cytometry we have quantified cellular levels of TERRA and TERC lncRNAs in culture cell lines and PBMCs. This easy-to-use method appointed RNA-Flow allows reliable lncRNA quantification with broad applications in basic research and clinical diagnostics. In addition, the staining protocol presented here was prove ...[more]
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