Drp1/Fis1-Dependent Pathologic Fission and Associated Damaged Extracellular Mitochondria Contribute to Macrophage Dysfunction in Endotoxin Tolerance.

ABSTRACT:

Objectives

Recent publications have shown that mitochondrial dynamics can govern the quality and quantity of extracellular mitochondria subsequently impacting immune phenotypes. This study aims to determine if pathologic mitochondrial fission mediated by Drp1/Fis1 interaction impacts extracellular mitochondrial content and macrophage function in sepsis-induced immunoparalysis.

Design

Laboratory investigation.

Setting

University laboratory.

Subjects

C57BL/6 and BALB/C mice.

Interventions

Using in vitro and murine models of endotoxin tolerance (ET), we evaluated changes in Drp1/Fis1-dependent pathologic fission and simultaneously measured the quantity and quality of extracellular mitochondria. Next, by priming mouse macrophages with isolated healthy mitoch

SUBMITTER: Mukherjee R 

PROVIDER: S-EPMC9133053 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature